# GSE133344 CRISPRa and combination calibration reference

## Permitted role

This study is registered as a cross-cell-line method-calibration reference for CRISPRa and combinatorial perturbation design.

- Context: K562, not skeletal muscle or DMD.
- Published study scale: 287 perturbations, including single and pair CRISPRa conditions.
- Current candidates present in the official filtered expression feature list: 20/21.
- Absent feature symbols: INTS13.

## Critical distinction

Expression-feature presence only means a transcript could be represented in the processed matrix. It does not establish that the gene was targeted by CRISPRa, nor does it authorize an overexpression prediction.

- Filtered-gene file SHA-256: `0dcba3cf4f3095b3fc1fa31b402c562bca7eea8d7d9ffd753e7b446dc37b9e3d`.

GSE293514 is a bounded human-myoblast fusion screen; GSE272233 is an orthogonal DMD-correction transcriptome reference; GSE52529 is an unperturbed, time-anchored myogenic trajectory reference; and GSE133344 is cross-cell-line CRISPRa/combination method calibration. None supplies direct independent DMD perturbation validation for the current 21 candidates, observed candidate-pair interactions, candidate overexpression responses in muscle, or perturbation-conditioned fate.